anti neun rabbit polyclonal antibody  (Proteintech)

Journal: Research

Article Title: Aerobic Exercise-Induced TGF-β Receptor Reprogramming Disrupts Neutrophil–Microglia Crosstalk to Attenuate Early Brain Injury after Subarachnoid Hemorrhage

doi: 10.34133/research.1301

Figure Lengend Snippet: Animal study verifying the effect of AE in mitigating EBI of SAH. (A) The complete timeline of an experimental plan, schematic diagram, and actual skull base image of the SAH/Sham model in this animal experiment. Some elements are created in BioRender (2026); https://BioRender.com/vwwir98 . (B) SAH grading score of each group in this stage. n = 24. (C) Modified Garcia score of each group, n = 24. (D) The water content of the left and right brains in different groups at this stage. n = 6. (E and F) Apoptosis of bleeding focus cortical neurons was detected by TUNEL assay (red), NeuN (green), and DAPI (blue) immunofluorescence. n = 6. Scale bars, 50 μm. (G) Bars show changes in BBs in SAH mice. n = 24. (H to N) Western blotting images and quantitative data of relative expression level of Il-1b, Il-6, Nos2, Il-4, Il-10, and Tgfb1 in the ipsilateral cortex after SAH. n = 6. (O) The coronal sections were stained with hematoxylin and eosin (H&E) for histological evaluation AE-regulated in SAH. n = 6. Scale bars, 50 μm. (P) Histopathological changes detected by Nissl’s staining in hippocampus and the hemorrhagic cortex. n = 6. Scale bars, 50 μm. In (B), (C), and (G), data were represented as median (interquartile range), and P values were calculated using Kruskal–Wallis H with Dunn test. In (D), (F), and (I) to (N), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including Z and P values for Kruskal–Wallis test and F and P values for ANOVAs, are provided in Data to .

Article Snippet: After fixation, the tissue was processed for TUNEL assay with the cell apoptosis detection kit (Servicebio, G1502-50T), and Rabbit anti-NeuN polyclonal antibody (ABclonal, A0951) and its corresponding secondary antibody: Donkey anti-rabbit IgG conjugated to Alexa Fluor 488 (Antgene, ANT024, diluted 1:400).

Techniques: Modification, TUNEL Assay, Immunofluorescence, Western Blot, Expressing, Staining

Journal: Frontiers in Medicine

Article Title: Gastrodia elata fermentation alleviates methamphetamine-induced neuroinflammation and anxiety- and depression-like behaviors by regulating the PI3K-AKT signaling pathway

doi: 10.3389/fmed.2026.1768944

Figure Lengend Snippet: FGE mitigates METH-induced neuronal loss in the mouse hippocampus. (A) Representative immunohistochemical images of NeuN expression in the hippocampus CA1, CA3, and DG regions across different groups. Red arrows indicate examples of pyknotic or damaged neurons. (B–D) Quantitative analysis of the number of NeuN-positive cells in the hippocampus CA1, CA3, and DG regions. * p < 0.05, ** p < 0.01, *** p < 0.001; # p < 0.05, ## p < 0.01, ### p < 0.001, & p < 0.05, && p < 0.01, &&& p < 0.001.

Article Snippet: SP Rabbit/Mouse HRP Kit (DAB) (Cat# SA00001-2/SA00001-1, CWBIO, China); DAB Substrate Kit (Cat# ZLI-9018, Zhongshan Jinqiao, Beijing, China); SPARKscript II RT Kit/SYBR Green RT-PCR Kit (Cat# AG0305-B/AH0104-B, SparkJade, China); Anti-Iba1 Rabbit Polyclonal Antibody (Cat# F155306, Abways, China); Anti-GFAP Rabbit Polyclonal Antibody (Cat# CY5424, Abcam, United Kingdom); Anti-NeuN Rabbit Polyclonal Antibody (Cat# 26975-1-AP, Proteintech, China); AKT/phosphorylated AKT (p-AKT)/phosphorylated PI3K (p-PI3K)/GAPDH (Cat# 10176-2-AP/66444-1-IG/20584-1-AP/60004-1-IG, Proteintech, China); PI3K (Cat# AF3242, Affinity, China).

Techniques: Immunohistochemical staining, Expressing

Journal: Experimental Biology and Medicine

Article Title: Curcumin-enhanced elvitegravir therapy mitigates neuroinflammation and cognitive deficits in EcoHIV mice

doi: 10.3389/ebm.2025.10758

Figure Lengend Snippet: Brain levels of 8-OHdG (A,B) and glutamate (C,D) were measured in EcoHIV-infected mice treated with EVG, CUR, or their combination (EVG + CUR) via IN or IP routes. (E–I) Western blot analysis was conducted to assess the expression of neural protein markers NeuN, TMEM119, synaptophysin, L1CAM, and GFAP in the mice brains. Data are expressed as mean ± SEM (n = 4 per group). Statistical analysis was performed by one-way ANOVA with Tukey’s post hoc test. *, **, and *** represents p ≤ 0.05, p ≤ 0.01, and p ≤ 0.001 respectively.

Article Snippet: Primary antibody incubation was carried out overnight at 4 °C using the following antibodies: NeuN rabbit polyclonal antibody (1:1000, Proteintech, Cat# 26975-1-AP), synaptophysin mouse monoclonal antibody (1:20,000, Proteintech, Cat# 67864-1-Ig), GFAP rabbit polyclonal antibody (1:1000), L1CAM rabbit polyclonal antibody (Proteintech, Cat# 20659-1-AP), and β-actin mouse monoclonal antibody (1:20,000, Proteintech, Cat# 66009-1-Ig) as an internal loading control.

Techniques: Infection, Western Blot, Expressing